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rabbit anti pparg antibodies  (Proteintech)


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    Proteintech rabbit anti pparg antibodies
    Rabbit Anti Pparg Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pparg antibodies/product/Proteintech
    Average 96 stars, based on 562 article reviews
    rabbit anti pparg antibodies - by Bioz Stars, 2026-03
    96/100 stars

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    Figure 3. IL-13 potentiates <t>PPARg-mediated</t> beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with
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    Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with

    Journal: Journal of Clinical Investigation

    Article Title: Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity

    doi: 10.1172/jci169152

    Figure Lengend Snippet: Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with

    Article Snippet: Five μg of chromatin was used for each ChIP reaction that included either the negative control normal rabbit IgG (Cell Signaling #2729), PPARg (81B8) rabbit mAb (Cell Signaling #2443), or acetyl-histone H3 (Lys27) (D5E4) XP rabbit mAb (Cell Signaling #8173).

    Techniques: Western Blot, Control, Quantitative RT-PCR

    Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase

    Journal: Journal of Clinical Investigation

    Article Title: Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity

    doi: 10.1172/jci169152

    Figure Lengend Snippet: Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase

    Article Snippet: Five μg of chromatin was used for each ChIP reaction that included either the negative control normal rabbit IgG (Cell Signaling #2729), PPARg (81B8) rabbit mAb (Cell Signaling #2443), or acetyl-histone H3 (Lys27) (D5E4) XP rabbit mAb (Cell Signaling #8173).

    Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation, Binding Assay, Luciferase